5MXY
KustC0563 c-type cytochrome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 47.820, 47.820, 99.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.140 - 1.900 |
| R-factor | 0.20041 |
| Rwork | 0.199 |
| R-free | 0.22978 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.289 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 9541 | 1329 |
| <I/σ(I)> | 12.2 | 1.54 |
| Completeness [%] | 97.7 | 98.7 |
| Redundancy | 6.9 | 6.9 |
| CC(1/2) | 0.823 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Zn-acetate, 0.1 M MES pH 6.0, 10% w/v PEG 8000 |
