5MXU
Structure of the Y503F mutant of vanillyl alcohol oxidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-15 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.999 |
Spacegroup name | I 4 |
Unit cell lengths | 138.012, 138.012, 132.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.940 - 2.800 |
R-factor | 0.17884 |
Rwork | 0.175 |
R-free | 0.24886 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vao |
RMSD bond length | 0.011 |
RMSD bond angle | 1.547 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.940 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.113 | 0.705 |
Rpim | 0.087 | 0.545 |
Number of reflections | 27846 | 4226 |
<I/σ(I)> | 7 | 1.3 |
Completeness [%] | 91.8 | 94.3 |
Redundancy | 2.4 | 2.4 |
CC(1/2) | 0.988 | 0.540 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG4000, sodium acetate |