5MXJ
Structure of the Y108F mutant of vanillyl alcohol oxidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-15 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.71, 2.25 |
Spacegroup name | I 4 |
Unit cell lengths | 137.900, 137.900, 132.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.910 - 2.800 |
R-factor | 0.18383 |
Rwork | 0.180 |
R-free | 0.25860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vao |
RMSD bond length | 0.011 |
RMSD bond angle | 1.561 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.910 | 2.800 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.098 | 0.711 |
Rpim | 0.077 | 0.580 |
Number of reflections | 27138 | 4095 |
<I/σ(I)> | 9 | 1.3 |
Completeness [%] | 89.7 | 91.9 |
Redundancy | 2.3 | 2.1 |
CC(1/2) | 0.985 | 0.539 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | PEG4000, sodium acetate |