5MXH
Photorhabdus asymbiotica lectin (PHL) in complex with D-galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-22 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.895 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 81.323, 81.323, 114.181 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.350 - 1.950 |
| R-factor | 0.18075 |
| Rwork | 0.179 |
| R-free | 0.21719 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mxe |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.491 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.350 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.099 | 0.790 |
| Number of reflections | 32451 | 4636 |
| <I/σ(I)> | 18.8 | 3.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.1 | 11.2 |
| CC(1/2) | 0.999 | 0.877 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | 3.7-4.3 M NaCl, 100 mM Hepes pH 7.5 |
