5MXE
Photorhabdus asymbiotica lectin (PHL) in free form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-17 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 81.235, 81.235, 113.441 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.160 - 1.900 |
| R-factor | 0.1676 |
| Rwork | 0.166 |
| R-free | 0.19101 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c9o |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.308 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.160 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.071 | 0.237 |
| Number of reflections | 34331 | 4583 |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 98.8 | 93.4 |
| Redundancy | 3.6 | 2.5 |
| CC(1/2) | 0.997 | 0.934 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | 3.7-4.3 M NaCl, 100 mM Hepes pH 7.5 |
