5MX8
Crystal structure of H. pylori purine nucleoside phosphorylase from clinical isolate HpPNP-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-06 |
| Detector | OXFORD RUBY CCD |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 143.596, 143.596, 143.596 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.311 - 2.400 |
| R-factor | 0.2158 |
| Rwork | 0.213 |
| R-free | 0.25910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mx4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.063 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.311 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 20243 |
| <I/σ(I)> | 15.2 |
| Completeness [%] | 99.4 |
| Redundancy | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 291 | 0.2M NaCl, 0.1M Na/K phosphate pH 6.2, 50% PEG 200 |
