5MX1

Crystal structure of human chondroadherin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.739
Spacegroup name	C 1 2 1
Unit cell lengths	215.450, 60.700, 57.660
Unit cell angles	90.00, 100.60, 90.00

Refinement procedure

Resolution	58.350 - 2.170
R-factor	0.1907
Rwork	0.188
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xku
RMSD bond length	0.003
RMSD bond angle	0.690
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.400	2.230
High resolution limit [Å]	2.170	2.170
Rmerge	0.148	0.840
Rpim	0.085	0.469
Number of reflections	37325
<I/σ(I)>	9.3
Completeness [%]	95.8	75.3
Redundancy	5.8	3.9
CC(1/2)	0.987	0.506

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	10 mg/ml protein + 200 mM monobasic potassium phosphate (pH 4.8), 20% (w/v) polyethylene glycol 3350