5MWL
INOSITOL 1,3,4,5,6-PENTAKISPHOSPHATE 2-KINASE FROM M. MUSCULUS IN COMPLEX WITH ATP and IP5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979490 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.623, 140.758, 68.662 |
| Unit cell angles | 90.00, 106.53, 90.00 |
Refinement procedure
| Resolution | 70.380 - 3.200 |
| R-factor | 0.22614 |
| Rwork | 0.225 |
| R-free | 0.24529 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mw8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.185 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 70.380 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 19318 |
| <I/σ(I)> | 14.7 |
| Completeness [%] | 99.3 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.25 | 291 | Magnesium chloride, MES, PEG 6000 |
