5MWF
Human Jagged2 C2-EGF2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.628, 92.929, 97.004 |
| Unit cell angles | 71.68, 83.16, 82.68 |
Refinement procedure
| Resolution | 38.009 - 2.800 |
| R-factor | 0.22 |
| Rwork | 0.217 |
| R-free | 0.27510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mw5 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.755 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.009 | 2.890 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.103 | 0.685 |
| Number of reflections | 49334 | |
| <I/σ(I)> | 7.3 | 1.3 |
| Completeness [%] | 97.6 | 97.7 |
| Redundancy | 2.2 | 2.3 |
| CC(1/2) | 0.990 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | PEG 8000, Ammonium sulphate, Sodium cacodylate |
