5MW0

Complex between the Leucine Zipper (LZ) and Centrosomin-motif 2 (CM2) domains of Drosophila melanogaster Centrosomin (Cnn) - L535E mutant form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97623
Spacegroup name	P 1 21 1
Unit cell lengths	45.769, 43.930, 68.640
Unit cell angles	90.00, 108.28, 90.00

Refinement procedure

Resolution	31.187 - 2.000
R-factor	0.2389
Rwork	0.237
R-free	0.26410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mvw
RMSD bond length	0.005
RMSD bond angle	0.574
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.190	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.068	0.317
Rpim	0.046	0.222
Number of reflections	17198	1246
<I/σ(I)>	6.1	1.8
Completeness [%]	97.2	97.6
Redundancy	3	3.1
CC(1/2)	0.992	0.521

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	294	15%(w/v) PEG4K, 0.2M imidazole malate pH7.0