5MVX

Human DLL4 C2-EGF3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92
Spacegroup name	C 1 2 1
Unit cell lengths	127.896, 49.753, 70.031
Unit cell angles	90.00, 109.15, 90.00

Refinement procedure

Resolution	66.155 - 2.170
R-factor	0.1847
Rwork	0.182
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cc1
RMSD bond length	0.005
RMSD bond angle	0.812
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.200	2.240
High resolution limit [Å]	2.170	2.170
Rmerge	0.054	0.508
Number of reflections	21802
<I/σ(I)>	14.6
Completeness [%]	98.0	91.6
Redundancy	4.5	3.7
CC(1/2)	0.998	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	PEG 20K, MES