5MVV
Crystal structure of Plasmodium falciparum actin I- gelsolin segment 1 -CdATP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2015-06-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9806 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 110.420, 68.920, 71.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.550 - 1.400 |
| R-factor | 0.12948 |
| Rwork | 0.129 |
| R-free | 0.15853 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.784 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.000 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 72676 |
| <I/σ(I)> | 33.81 |
| Completeness [%] | 100.0 |
| Redundancy | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.9 | 293.15 | 14 or 15% PEG3350, 0.2M potassium thiocyanate, 0.1M bis-tris pH 5.9. Drops were streak-seeded from crystals with calcium. |
