5MVT
Crystal structure of an A-DNA dodecamer featuring an alternating pyrimidine-purine sequence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-21 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.06 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 44.288, 44.288, 57.841 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.355 - 1.896 |
| R-factor | 0.2031 |
| Rwork | 0.202 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mvl |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.177 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 38.355 |
| High resolution limit [Å] | 1.896 |
| Number of reflections | 5540 |
| <I/σ(I)> | 21 |
| Completeness [%] | 99.9 |
| Redundancy | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | Sodium chloride (12 mM) Potassium chloride (80 mM) Hexammine cobalt(III) chloride (2 mM) MPD (45% v/v) |
