5MVP
Crystal structure of an A-DNA dodecamer containing the GGGCCC motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.976 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 43.256, 43.256, 61.552 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.461 - 1.606 |
R-factor | 0.1557 |
Rwork | 0.153 |
R-free | 0.20890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mvl |
RMSD bond length | 0.014 |
RMSD bond angle | 1.190 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 37.461 |
High resolution limit [Å] | 1.606 |
Number of reflections | 9111 |
<I/σ(I)> | 23 |
Completeness [%] | 99.7 |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Sodium chloride (80 mM) Potassium chloride (12 mM) Magnesium chloride (20 mM) Spermine tetrahydrochloride (12 mM) Sodium cacodylate (40 mM, pH 7.0) MPD (45% v/v) |