5MVI
Crystal structure of 2-methylcitrate dehydratase (PrpD) from Salmonella enterica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9282 |
| Spacegroup name | H 3 |
| Unit cell lengths | 139.662, 139.662, 513.484 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.140 - 3.050 |
| R-factor | 0.21437 |
| Rwork | 0.213 |
| R-free | 0.23870 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.419 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.140 |
| High resolution limit [Å] | 3.050 |
| Number of reflections | 70843 |
| <I/σ(I)> | 5.7 |
| Completeness [%] | 99.5 |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2M Sodium fluoride 20% w/vPEG 3350 |
