5MVH
Glycoside Hydrolase BACCELL_00856
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-07-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.842, 68.627, 52.880 |
| Unit cell angles | 90.00, 97.72, 90.00 |
Refinement procedure
| Resolution | 52.400 - 1.800 |
| R-factor | 0.16905 |
| Rwork | 0.167 |
| R-free | 0.21352 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.434 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 68.600 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 112843 |
| <I/σ(I)> | 12 |
| Completeness [%] | 98.4 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 16% PEG4000, Sodium acetate 0.1M, pH 4.6 |
