5MVG
Crystal structure of non-amyloidogenic light chain dimer M7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2016-02-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 70.721, 70.721, 171.953 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.970 - 2.200 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5m6i |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.050 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.700 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.077 | 0.536 |
| Number of reflections | 26134 | 2224 |
| <I/σ(I)> | 26 | 4.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.9 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 0.1 M MMT (Malic acid, MES and Tris-base buffer) pH 4.0, 25% w/v PEG 1.5K |
