5MV7

Structure of human Myosin 7b C-terminal MyTH4-FERM MF2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978570
Spacegroup name	C 1 2 1
Unit cell lengths	123.670, 42.810, 118.370
Unit cell angles	90.00, 97.69, 90.00

Refinement procedure

Resolution	35.170 - 2.440
R-factor	0.211
Rwork	0.209
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.530
High resolution limit [Å]	2.440	2.440
Number of reflections	22640
<I/σ(I)>	11.86	11.86
Completeness [%]	97.4	93.7
Redundancy	3.4
CC(1/2)	0.997	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	290	31-34% (v/v) PEG 400, 100 mM Tris-HCl pH 8.5 and 200 mM Li2SO4