5MV3
ACC1 Fab fragment in complex with CII583-591 (CG10)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 |
| Unit cell lengths | 87.353, 92.844, 121.706 |
| Unit cell angles | 92.50, 106.53, 104.31 |
Refinement procedure
| Resolution | 50.010 - 2.950 |
| R-factor | 0.20442 |
| Rwork | 0.202 |
| R-free | 0.26098 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mu0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.471 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 3.010 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.073 | 0.511 |
| Number of reflections | 73522 | 4569 |
| <I/σ(I)> | 11.4 | 1.8 |
| Completeness [%] | 99.0 | 98.6 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% (w/v) PEG3350, 0.1 M Bistris-Propane pH 7.0, 0.2 M sodium acetate |
