5MV2
Crystal structure of the E protein of the Japanese encephalitis live attenuated vaccine virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97621 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.600, 55.900, 90.100 |
| Unit cell angles | 90.00, 132.10, 90.00 |
Refinement procedure
| Resolution | 19.944 - 2.100 |
| R-factor | 0.1824 |
| Rwork | 0.180 |
| R-free | 0.22250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mv1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.185 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.144 | |
| Number of reflections | 28195 | |
| <I/σ(I)> | 9.7 | 2.1 |
| Completeness [%] | 98.7 | 98.7 |
| Redundancy | 6.7 | 7 |
| CC(1/2) | 0.995 | 0.877 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 291 | 0.2M Tri-Na citrate, pH 7.9, 18% PEG 3350 |
