5MV1
Crystal structure of the E protein of the Japanese encephalitis virulent virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.090, 33.190, 98.510 |
| Unit cell angles | 90.00, 107.46, 90.00 |
Refinement procedure
| Resolution | 19.746 - 2.250 |
| R-factor | 0.2137 |
| Rwork | 0.211 |
| R-free | 0.27240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p54 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.777 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 21345 | |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 4.4 | 4.6 |
| CC(1/2) | 0.990 | 0.380 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 0.1M Tris-HCI, pH 8.0, 16% PEG 1000, 0.3M ammonium acetate |
