5MUK
Glycoside Hydrolase BT3686
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.01 |
| Spacegroup name | P 61 |
| Unit cell lengths | 119.878, 119.878, 52.432 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.020 - 1.490 |
| R-factor | 0.17729 |
| Rwork | 0.176 |
| R-free | 0.20195 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.511 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 24.020 |
| High resolution limit [Å] | 1.490 |
| Number of reflections | 311879 |
| <I/σ(I)> | 15.2 |
| Completeness [%] | 100.0 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20 % PEG3350 and 200 mM CH3CO2K, with 0.3 M Rhamnose |
