5MU7
Crystal Structure of the beta/delta-COPI Core Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976251 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 137.591, 177.472, 62.722 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.072 - 2.570 |
| R-factor | 0.1744 |
| Rwork | 0.172 |
| R-free | 0.22870 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.890 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.100 | 2.660 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.068 | |
| Rpim | 0.644 | |
| Number of reflections | 24909 | |
| <I/σ(I)> | 15.1 | 1.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.6 | 6.5 |
| CC(1/2) | 0.999 | 0.502 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | protein concentration 13 mg/ml reservoir composition: 0.2 M magnesium formate 0.1 M Tris pH 7.0 24 % PEG3350 drop composition: 400 nl protein + 400 nl reservoir |
