5MU4
Tail Tubular Protein A of Klebsiella pneumoniae bacteriophage KP32
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 63 |
| Unit cell lengths | 138.320, 138.320, 102.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.950 - 1.900 |
| R-factor | 0.1841 |
| Rwork | 0.183 |
| R-free | 0.22960 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.957 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.150 | 1.900 |
| Rmerge | 0.107 | 0.081 | |
| Rmeas | 0.113 | 0.086 | |
| Rpim | 0.040 | 0.028 | 0.788 |
| Total number of observations | 718685 | ||
| Number of reflections | 87540 | ||
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 100.0 | 99.8 | 99.8 |
| Redundancy | 8.2 | 10.6 | 6.7 |
| CC(1/2) | 0.987 | 0.643 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 35% Tacsimate | |
| 1 | VAPOR DIFFUSION | 293 | 35% Tacsimate |
