5MU4

Tail Tubular Protein A of Klebsiella pneumoniae bacteriophage KP32

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 63
Unit cell lengths	138.320, 138.320, 102.930
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.950 - 1.900
R-factor	0.1841
Rwork	0.183
R-free	0.22960
Structure solution method	SAD
RMSD bond length	0.021
RMSD bond angle	1.957
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.930
High resolution limit [Å]	1.900	5.150	1.900
Rmerge	0.107	0.081
Rmeas	0.113	0.086
Rpim	0.040	0.028	0.788
Total number of observations	718685
Number of reflections	87540
<I/σ(I)>	9.4
Completeness [%]	100.0	99.8	99.8
Redundancy	8.2	10.6	6.7
CC(1/2)		0.987	0.643

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	35% Tacsimate
1	VAPOR DIFFUSION		293	35% Tacsimate