5MTU

Maltodextrin binding protein MalE1 from L. casei BL23 bound to alpha-cyclodextrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-28
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 21 21 21
Unit cell lengths	54.320, 82.390, 85.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.680 - 0.999
R-factor	0.131
Rwork	0.130
R-free	0.14300
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.050
Data reduction software	XDS (VERSION November 3, 2014)
Data scaling software	XDS (VERSION November 3, 2014)
Phasing software	SHELXDE (2013/2)
Refinement software	PHENIX ((1.10.1-2155_1168: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.810	1.035
High resolution limit [Å]	0.999	0.999
Rmerge	0.071	0.932
Number of reflections	203886
<I/σ(I)>	16.7	2
Completeness [%]	98.6	95.9
Redundancy	11.2	7.03

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	0.1 mM Na-Hepes, pH 7.0, 20-25% Jeffamine ED2003, pH 7.0, 5 mM alpha-cyclodextrin