5MTJ

Yes1-SH2 in complex with monobody Mb(Yes_1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-20
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.97919
Spacegroup name	I 41 2 2
Unit cell lengths	101.864, 101.864, 139.618
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.308 - 1.949
R-factor	0.1918
Rwork	0.190
R-free	0.22310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3K2M and 4TZI
RMSD bond length	0.008
RMSD bond angle	0.872
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.949	1.950
Rmerge	0.127
Rmeas	0.131
Rpim	0.040	0.544
Number of reflections	27106
<I/σ(I)>	24.44	1.5
Completeness [%]	99.8	99.7
Redundancy	19.1	16.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.25	291	2M Ammonium sulfate, 0.2M Lithium sulfate, 0.1M CAPS/NaOH pH10.25