5MTH
Structure of DC8E8 Fab at pH 6.5 crystallized in spacegroup P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.450, 111.770, 95.640 |
| Unit cell angles | 90.00, 90.56, 90.00 |
Refinement procedure
| Resolution | 38.860 - 1.730 |
| R-factor | 0.1876 |
| Rwork | 0.185 |
| R-free | 0.23550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 4OZ4 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.898 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.860 | 1.760 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmeas | 0.211 | 3.860 |
| Number of reflections | 88801 | |
| <I/σ(I)> | 7.98 | 0.4 |
| Completeness [%] | 98.0 | 81.3 |
| Redundancy | 5.6 | 4.4 |
| CC(1/2) | 0.108 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 20% PEG 8000, 0.1 M Na-cacodylate, 0.2 M Mg-acetate |
