5MSK
Mouse PA28beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.840, 132.610, 90.930 |
| Unit cell angles | 90.00, 92.19, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.600 |
| R-factor | 0.24269 |
| Rwork | 0.242 |
| R-free | 0.26311 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5msj |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.640 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.700 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.091 | 0.563 |
| Number of reflections | 23433 | |
| <I/σ(I)> | 9.6 | 2.3 |
| Completeness [%] | 97.7 | 99.5 |
| Redundancy | 3.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.1 M MgCl2, 0.1 M MES, 30 % PEG400 |
