5MRJ
Crystal structure of Endo-1,4-beta-xylanase-like protein from Acremonium chrysogenum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.96770 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 88.210, 124.828, 165.575 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.809 - 2.700 |
| R-factor | 0.224 |
| Rwork | 0.221 |
| R-free | 0.28480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b30 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.084 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Number of reflections | 25746 | |
| <I/σ(I)> | 21 | |
| Completeness [%] | 94.3 | 96.6 |
| Redundancy | 3.95 | 3.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 15% PEG 4K, 50 mM Sodium Citrate, 20 mM Sodium chloride, 20 mM Ammonium Sulfate |
