5MR8
Crystal structure of TRIM33 PHD-Bromodomain isoform B in complex with H3K9ac histone peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.885, 57.994, 71.488 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.997 - 1.740 |
| R-factor | 0.2299 |
| Rwork | 0.227 |
| R-free | 0.28620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u5m |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.792 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.250 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.035 | 0.678 |
| Number of reflections | 22066 | |
| <I/σ(I)> | 19.7 | 2.1 |
| Completeness [%] | 98.7 | 96 |
| Redundancy | 5.8 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 0.1 M Tris pH 8.5, 25% PEG3350 |
