5MR7
Crystal structure of the DBD domain of human Grhl2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.193, 48.274, 101.304 |
| Unit cell angles | 90.00, 102.92, 90.00 |
Refinement procedure
| Resolution | 43.370 - 2.500 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mph |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.370 | 2.586 |
| High resolution limit [Å] | 2.497 | 2.497 |
| Rmerge | 0.105 | 0.910 |
| Number of reflections | 17882 | |
| <I/σ(I)> | 11.01 | 1.7 |
| Completeness [%] | 99.0 | 97.61 |
| Redundancy | 4.4 | 4.3 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | reservoir with 21% PEG 3350, 0.2 M sodium formate |
