5MQP
Glycoside hydrolase BT_1002
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 208.230, 133.238, 224.077 |
| Unit cell angles | 90.00, 97.67, 90.00 |
Refinement procedure
| Resolution | 49.160 - 2.000 |
| R-factor | 0.18462 |
| Rwork | 0.183 |
| R-free | 0.22397 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3WKX and 4QJY |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.634 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.160 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.072 | 0.914 |
| Number of reflections | 406952 | |
| <I/σ(I)> | 9.8 | 1.4 |
| Completeness [%] | 99.8 | 70.8 |
| Redundancy | 3.9 | 3.6 |
| CC(1/2) | 0.997 | 0.708 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 10% (w/v) PEG 3350, 0.1 M Hepes pH 7.5 and 0.2 M L-Proline |
