5MQO
Glycoside hydrolase BT_1003
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.775 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 219.440, 219.440, 91.809 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 109.720 - 2.500 |
| R-factor | 0.19647 |
| Rwork | 0.194 |
| R-free | 0.23899 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wkx 4qjy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.592 |
| Data reduction software | DIALS (5.8.0155) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 109.720 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.133 | 0.954 |
| Number of reflections | 29265 | |
| <I/σ(I)> | 5.2 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.9 | 4.9 |
| CC(1/2) | 0.992 | 0.339 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 20% (w/v) PEG 3350 and 0.2 M potassium acetate |
