5MQN
Glycoside hydrolase BT_0986
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.913 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.291, 85.758, 120.870 |
| Unit cell angles | 90.00, 98.78, 90.00 |
Refinement procedure
| Resolution | 49.170 - 1.900 |
| R-factor | 0.17543 |
| Rwork | 0.174 |
| R-free | 0.20512 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.523 |
| Data reduction software | XDS (5.8.0155) |
| Data scaling software | Aimless |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.170 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.076 | 0.653 |
| Number of reflections | 101961 | |
| <I/σ(I)> | 9.7 | 1.7 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 3.7 | 3.2 |
| CC(1/2) | 0.996 | 0.671 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 15% (w/v) PEG 550 MME, 15% (w/v) PEG 20000, 0.25 M rahmnose, 50mM hepes and 50 mm MOPS pH 7.5 |
