5MQM
Glycoside hydrolase BT_0986
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.050, 84.810, 120.630 |
| Unit cell angles | 90.00, 99.20, 90.00 |
Refinement procedure
| Resolution | 50.690 - 2.110 |
| R-factor | 0.20064 |
| Rwork | 0.199 |
| R-free | 0.24026 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | apo model |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.502 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.690 | 2.160 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.088 | 0.818 |
| Number of reflections | 75602 | |
| <I/σ(I)> | 9.9 | 1.5 |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.995 | 0.548 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 15% (w/v) PEG 550 MME, 15% (w/v) PEG 20000, 0.25 M rahmnose, 50mM hepes and 50 mm MOPS pH 7.56 6mM D-rhamnopyranose tetrazole |
