5MQH
Structure of the Phosphatase Domain of the Cell Fate Determinant SpoIIE from Bacillus subtilis in a crystal form without domain swapping
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 56.285, 122.506, 81.617 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.990 - 2.450 |
| R-factor | 0.21386 |
| Rwork | 0.211 |
| R-free | 0.27751 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t9l |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.648 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.340 | 2.480 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Number of reflections | 10961 | |
| <I/σ(I)> | 20.1 | 2.8 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 6.3 | 6.2 |
| CC(1/2) | 0.999 | 0.874 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 2M Sodium formate, 0.1M Sodium acetate |
