5MQ9

Crystal structure of Rae1 (YacP) from Bacillus subtilis (W164L mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-08
Detector	ADSC QUANTUM 270
Wavelength(s)	1.30510
Spacegroup name	P 21 21 21
Unit cell lengths	57.700, 63.950, 138.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.996 - 3.174
R-factor	0.2828
Rwork	0.281
R-free	0.32020
Structure solution method	SIRAS
RMSD bond length	0.003
RMSD bond angle	0.498
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	autoSHARP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.170
Number of reflections	9099
<I/σ(I)>	12.11
Completeness [%]	100.0
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	293	0.05 M sodium acetate, 2M ammonium sulfate