5MQ8

Crystal structure of Rae1 (YacP) from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.873
Spacegroup name	P 21 21 21
Unit cell lengths	53.628, 83.669, 95.729
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.870 - 2.250
R-factor	0.2083
Rwork	0.207
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.030	0.290
Number of reflections	21060
<I/σ(I)>	17.77	2.68
Completeness [%]	100.0	100
Redundancy	2	2
CC(1/2)	0.999	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	Protein at 6 mg/mL in 0.07 M Tris-HCl pH 6.5, 5.2% PEG 8000, 35% glycerol