5MPZ
Crystal structure of CREBBP bromodomain complexed with CBP007
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-21 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999998 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.057, 44.658, 60.211 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.869 - 1.400 |
R-factor | 0.1587 |
Rwork | 0.158 |
R-free | 0.17630 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.957 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11_2567: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 44.060 |
High resolution limit [Å] | 1.400 |
Rmerge | 0.044 |
Number of reflections | 23766 |
<I/σ(I)> | 21.2 |
Completeness [%] | 98.9 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M MgCl2, 0.1 M Bis-Tris, pH 6.5, 5% v/v ethylene glycol, 23% PEG3350 |