5MPY

Crystal structure of Arabidopsis thaliana RNA editing factor MORF9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-19
Detector	PSI PILATUS 6M
Wavelength(s)	0.918
Spacegroup name	P 65 2 2
Unit cell lengths	73.875, 73.875, 264.938
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.016 - 2.247
R-factor	0.1973
Rwork	0.195
R-free	0.23810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mpw
RMSD bond length	0.009
RMSD bond angle	0.979
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.247	2.250
Number of reflections	21313
<I/σ(I)>	16.99	1.56
Completeness [%]	99.6	99
Redundancy	10.5	10.8
CC(1/2)		0.772

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.08 M sodium cacodylate, pH 6.5, 14.4% (w/v) PEG 8000, 20% (v/v) glycerol, 0.16 M calcium acetate and 0.04 M NaCl