5MPW
Crystal structure of Arabidopsis thaliana RNA editing factor MORF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.916 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.280, 44.940, 68.200 |
| Unit cell angles | 98.10, 91.98, 111.96 |
Refinement procedure
| Resolution | 37.982 - 1.499 |
| R-factor | 0.1591 |
| Rwork | 0.157 |
| R-free | 0.19230 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.240 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | autoSHARP |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.499 | 1.500 |
| Number of reflections | 66951 | |
| <I/σ(I)> | 10.3 | 1.5 |
| Completeness [%] | 97.6 | 94 |
| Redundancy | 6.5 | 3.6 |
| CC(1/2) | 0.563 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.1 M Tris, pH 8.0 2.8 M NH4SO4 |
