5MPH

Structural Basis of Gene Regulation by the Grainyhead Transcription Factor Superfamily

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.5
Spacegroup name	P 61 2 2
Unit cell lengths	106.752, 106.752, 115.634
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.020 - 2.337
R-factor	0.1935
Rwork	0.192
R-free	0.22930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.054
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.020	2.421
High resolution limit [Å]	2.337	2.337
Rmerge	0.060	1.918
Number of reflections	17043
<I/σ(I)>	35.62	1.88
Completeness [%]	100.0	99.88
Redundancy	24.9	24.3
CC(1/2)	1.000	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	2.1 M (NH4)2SO4 and 0.1 M NaOAc, pH 5.5