5MP5

Crystal structure of DC8E8 Fab in the complex with a 14-mer tau peptide at pH 6.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-27
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	60.750, 82.120, 89.040
Unit cell angles	92.35, 95.36, 89.86

Refinement procedure

Resolution	34.500 - 2.310
R-factor	0.2368
Rwork	0.235
R-free	0.27650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 4OZ4
RMSD bond length	0.010
RMSD bond angle	1.521
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.500	34.500	2.460
High resolution limit [Å]	2.310	10.730	2.400
Rmerge	0.048	0.018	0.338
Number of reflections	64648
<I/σ(I)>	14.86	43.58	2.54
Completeness [%]	96.6	87.3	96.4
Redundancy	2.095	2.055	2.047
CC(1/2)	0.997	0.999	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	20% PEG 8000, 0.1 M Na-cacodylate, 0.2 M Mg-acetate