5MOV
Crystal structure of Ck2alpha with ZT0633 bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-27 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.149, 45.356, 63.154 |
| Unit cell angles | 90.00, 111.67, 90.00 |
Refinement procedure
| Resolution | 35.890 - 2.200 |
| R-factor | 0.2088 |
| Rwork | 0.206 |
| R-free | 0.26880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cvh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | XPREP |
| Data scaling software | XPREP |
| Phasing software | PHASER |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.890 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.145 | 0.762 |
| Number of reflections | 15457 | |
| <I/σ(I)> | 10.59 | 1.15 |
| Completeness [%] | 98.0 | 94.7 |
| Redundancy | 8 | 4.23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 107mM Mes pH 6.5, 29% glycerol ethoxylate, 1 M ammonium acetate |
