5MOM

Crystal Structure of PCNA encoding the hypomorphic mutation S228I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97625
Spacegroup name	P 41 21 2
Unit cell lengths	162.950, 162.950, 140.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	89.069 - 2.270
R-factor	0.1996
Rwork	0.199
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vym
RMSD bond length	0.008
RMSD bond angle	1.126
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.070	2.330
High resolution limit [Å]	2.270	2.270
Number of reflections	87453
<I/σ(I)>	17.7	1.5
Completeness [%]	100.0	100
Redundancy	13.4	13.2
CC(1/2)		0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.1M NaAcO, 2M (NH4)2SO4