5MOA
ABA RECEPTOR FROM TOMATO, SlPYL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 89.493, 89.493, 51.941 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.147 - 1.650 |
| R-factor | 0.2148 |
| Rwork | 0.214 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.083 |
| Data scaling software | Aimless (v7.0) |
| Phasing software | MOLREP (v7.0) |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.150 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 281445 | |
| <I/σ(I)> | 26.5 | |
| Completeness [%] | 96.5 | 99.4 |
| Redundancy | 7.5 | 7.7 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 295 | 3.5 M de sulfato de amonio pH 7.0 |
