5MNT
Bacteriophage Qbeta maturation protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 112.789, 232.515, 353.473 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 176.740 - 3.320 |
| R-factor | 0.23073 |
| Rwork | 0.228 |
| R-free | 0.27936 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.368 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 176.740 | 3.520 |
| High resolution limit [Å] | 3.320 | 3.320 |
| Rmerge | 0.176 | 0.913 |
| Number of reflections | 67077 | |
| <I/σ(I)> | 8.74 | 1.42 |
| Completeness [%] | 97.1 | 95.1 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.983 | 0.518 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 40 mM potassium dihydrogen phosphate, 17% PEG 8000, 20% glycerol |
