5MN8

S. aureus FtsZ 12-316 F138A GTP Closed form (5FCm)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9282
Spacegroup name	P 31 2 1
Unit cell lengths	69.781, 69.781, 295.465
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.773 - 3.500
R-factor	0.2673
Rwork	0.265
R-free	0.31370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mn5
RMSD bond length	0.002
RMSD bond angle	0.575
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.600
High resolution limit [Å]	3.500	3.500
Rmerge	0.146	1.226
Number of reflections	10823
<I/σ(I)>	6.8	1.4
Completeness [%]	98.3	99.6
Redundancy	4.8	5.1
CC(1/2)	0.998	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.7	292	5 mg/ml; 1.6 M ammmonium sulfate, 0.5 M LiCl