5MN5

S. aureus FtsZ 12-316 T66W GTP Closed form (2TCm)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	43.781, 59.850, 187.664
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.245 - 2.802
R-factor	0.2191
Rwork	0.217
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wgl
RMSD bond length	0.006
RMSD bond angle	0.946
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.109	0.554
Number of reflections	12051
<I/σ(I)>	11.4	3
Completeness [%]	98.2	95.5
Redundancy	4.4	4.3
CC(1/2)	0.995	0.781

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	292	10 mg/ml; LiCl 1.136 M, PEG 6,000 31.4 %, 0.1 M MES pH 6