5MN4

S. aureus FtsZ 12-316 F138A GDP Open form (1FOf)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.93
Spacegroup name	C 1 2 1
Unit cell lengths	71.839, 51.116, 88.132
Unit cell angles	90.00, 110.97, 90.00

Refinement procedure

Resolution	41.147 - 1.500
R-factor	0.1798
Rwork	0.178
R-free	0.21190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vo8
RMSD bond length	0.009
RMSD bond angle	1.158
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.045	0.652
Number of reflections	45872
<I/σ(I)>	14.1	1.7
Completeness [%]	98.5	97.9
Redundancy	3.4	3.4
CC(1/2)	0.999	0.740

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	12.5 % w/v PEG 1k, 12.5 % w/v PEG 3350, 12.5 % v/v MPD, 0.1 M bicine/Tris pH 8.5, 0.03 M NaF, NaBr and NaI